d 180a stimulator Search Results


90
Digitimer Ltd electrical stimulator d 180a
Electrical Stimulator D 180a, supplied by Digitimer Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrical stimulator d 180a/product/Digitimer Ltd
Average 90 stars, based on 1 article reviews
electrical stimulator d 180a - by Bioz Stars, 2026-05
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90
Avanti Inc 18:0a/20:4-pe
Activation of macrophages induces externalization of 12-HETE-PEs on the surface of macrophages. Murine macrophages were activated with ionophore and externally exposed PE biotinylated and detected as described under “Experimental Procedures.” A , LC/MS/MS of biotinylated <t>18:0a/20:4-PE-biotin</t> from platelets. Lipid extracts from activated platelets derivatized with Sulfo-NHS-biotin were separated using LC/MS/MS as described under “Experimental Procedures,” monitoring for MRM 992/303. B , biotinylated 12-HETE-PEs were determined as described under “Experimental Procedures,” before and after ionophore activation of wild-type or 12/15-LOX −/− peritoneal macrophages ( n = 3, mean ± S.E.). C , biotinylated native PEs were determined as for 12-HETE-PEs, above ( n = 3, mean ± S.E.).
18:0a/20:4 Pe, supplied by Avanti Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/18:0a/20:4-pe/product/Avanti Inc
Average 90 stars, based on 1 article reviews
18:0a/20:4-pe - by Bioz Stars, 2026-05
90/100 stars
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90
Avanti Inc 18:0a/15-hete-pe
15-HETE-PE inhibits LPS-stimulated cytokine generation by human monocytes. Human monocytes were incubated with <t>18:0a/15-HETE-PE</t> or 18:0a/20:4-PE (20 or 40 ng/500 ul) with or without 10 ng/ml lipopolysaccharide (LPS) for 24 h. The supernatant was then analyzed using enzyme-linked immunosorbent assay kits for tumor necrosis factor-α ( A ) or GCSF ( B ).
18:0a/15 Hete Pe, supplied by Avanti Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/18:0a/15-hete-pe/product/Avanti Inc
Average 90 stars, based on 1 article reviews
18:0a/15-hete-pe - by Bioz Stars, 2026-05
90/100 stars
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Activation of macrophages induces externalization of 12-HETE-PEs on the surface of macrophages. Murine macrophages were activated with ionophore and externally exposed PE biotinylated and detected as described under “Experimental Procedures.” A , LC/MS/MS of biotinylated 18:0a/20:4-PE-biotin from platelets. Lipid extracts from activated platelets derivatized with Sulfo-NHS-biotin were separated using LC/MS/MS as described under “Experimental Procedures,” monitoring for MRM 992/303. B , biotinylated 12-HETE-PEs were determined as described under “Experimental Procedures,” before and after ionophore activation of wild-type or 12/15-LOX −/− peritoneal macrophages ( n = 3, mean ± S.E.). C , biotinylated native PEs were determined as for 12-HETE-PEs, above ( n = 3, mean ± S.E.).

Journal: The Journal of Biological Chemistry

Article Title: Phosphatidylethanolamine-esterified Eicosanoids in the Mouse

doi: 10.1074/jbc.M109.021634

Figure Lengend Snippet: Activation of macrophages induces externalization of 12-HETE-PEs on the surface of macrophages. Murine macrophages were activated with ionophore and externally exposed PE biotinylated and detected as described under “Experimental Procedures.” A , LC/MS/MS of biotinylated 18:0a/20:4-PE-biotin from platelets. Lipid extracts from activated platelets derivatized with Sulfo-NHS-biotin were separated using LC/MS/MS as described under “Experimental Procedures,” monitoring for MRM 992/303. B , biotinylated 12-HETE-PEs were determined as described under “Experimental Procedures,” before and after ionophore activation of wild-type or 12/15-LOX −/− peritoneal macrophages ( n = 3, mean ± S.E.). C , biotinylated native PEs were determined as for 12-HETE-PEs, above ( n = 3, mean ± S.E.).

Article Snippet: Cells were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (Avanti, AL), with or without 10 ng/ml LPS for 24 h. The supernatant was then removed and spun at 800 × g for 5 min. Cytokine generation was analyzed using enzyme-linked immunosorbent assay kits for granulocyte colony-stimulating factor or tumor necrosis factor-α (R&D).

Techniques: Activation Assay, Liquid Chromatography with Mass Spectroscopy

15-HETE-PE inhibits LPS-stimulated cytokine generation by human monocytes. Human monocytes were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (20 or 40 ng/500 ul) with or without 10 ng/ml lipopolysaccharide (LPS) for 24 h. The supernatant was then analyzed using enzyme-linked immunosorbent assay kits for tumor necrosis factor-α ( A ) or GCSF ( B ).

Journal: The Journal of Biological Chemistry

Article Title: Phosphatidylethanolamine-esterified Eicosanoids in the Mouse

doi: 10.1074/jbc.M109.021634

Figure Lengend Snippet: 15-HETE-PE inhibits LPS-stimulated cytokine generation by human monocytes. Human monocytes were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (20 or 40 ng/500 ul) with or without 10 ng/ml lipopolysaccharide (LPS) for 24 h. The supernatant was then analyzed using enzyme-linked immunosorbent assay kits for tumor necrosis factor-α ( A ) or GCSF ( B ).

Article Snippet: Cells were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (Avanti, AL), with or without 10 ng/ml LPS for 24 h. The supernatant was then removed and spun at 800 × g for 5 min. Cytokine generation was analyzed using enzyme-linked immunosorbent assay kits for granulocyte colony-stimulating factor or tumor necrosis factor-α (R&D).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

15-HETE-PE inhibits LPS-stimulated cytokine generation by human monocytes. Human monocytes were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (20 or 40 ng/500 ul) with or without 10 ng/ml lipopolysaccharide (LPS) for 24 h. The supernatant was then analyzed using enzyme-linked immunosorbent assay kits for tumor necrosis factor-α ( A ) or GCSF ( B ).

Journal: The Journal of Biological Chemistry

Article Title: Phosphatidylethanolamine-esterified Eicosanoids in the Mouse

doi: 10.1074/jbc.M109.021634

Figure Lengend Snippet: 15-HETE-PE inhibits LPS-stimulated cytokine generation by human monocytes. Human monocytes were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (20 or 40 ng/500 ul) with or without 10 ng/ml lipopolysaccharide (LPS) for 24 h. The supernatant was then analyzed using enzyme-linked immunosorbent assay kits for tumor necrosis factor-α ( A ) or GCSF ( B ).

Article Snippet: Cells were incubated with 18:0a/15-HETE-PE or 18:0a/20:4-PE (Avanti, AL), with or without 10 ng/ml LPS for 24 h. The supernatant was then removed and spun at 800 × g for 5 min. Cytokine generation was analyzed using enzyme-linked immunosorbent assay kits for granulocyte colony-stimulating factor or tumor necrosis factor-α (R&D).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay